Zika Virus: Prevention and Control

ZIKA, a mosquito borne virus is a flavivirus first identified in monkeys in Zika forest in Uganda in the year 1947. Thereafter, sporadic human cases have been reported in parts of Africa, the Americas, South East Asia and Western Pacific. According to Indian Council of Medical Research (ICMR) in 2007, first outbreak of Zika was documented from the Pacific region. Subsequently, outbreaks have been reported from Africa, America, Asia, French Polynesia, Western Pacific and Brazil. Since 2014, indigenous circulation of Zika virus (ZIKV) has been detected in the Americas. Alarm bells started ringing in October, 2015 in Brazil, when doctors saw a huge increase in babies born with microcephaly an abnormally small head often with consequent brain damage. Zika was found in the amniotic fluid of women carrying fetuses with microcephaly. According to World Health Organization (WHO) has reported 22 countries and territories in Americas from where local transmission of Zika virus has been reported. Microcephaly in the newborn and other neurological syndromes (Guillain Barre Syndrome) has been found temporarily associated with Zika virus infection. However, there are a number of genetic and other causes for microcephaly and neurological syndromes like Guillain Barre Syndrome. Zika virus disease has the potential for further international spread given the wide geographical distribution of the mosquito vector, a lack of immunity among population in newly affected areas and the high volume of international travel. As of now, the disease has not been reported in India. However, the mosquito that transmits Zika virus, namely Aedes aegypti , that also transmits dengue virus, is widely prevalent in India. The Zika virus is transmitted by the bite of infected Aedes aegypti mosquitoes, which is also the vector of dengue and chikungunya. However, Aedes albopictus could also be responsible. Incubation period is unknown. There are few reports of spread of Zika virus through infected blood and semen. However, the claims still need to be validated. However, in endemic regions, the usual recommendations for safe transfusions should be followed.

Aedes aegypti and Aedes albopictus

Clinical picture And Complications:
Only one in four infected patients develop symptoms of Zika disease. Symptoms are mild in adults and resemble the clinical manifestations of Dengue and Chikungunya. Most often cases of Zika are missed or misdiagnosed. Mild fever, malaise, rashes, joint fever, conjunctivitis are predominant symptoms which usually subside between 2-7 days of onset of illness. Though little is known about the complications of Zika virus disease, however, in subsequent outbreaks due to the virus in Brazil and French Polynesia, increased number in neurological and autoimmune problems including Gullain Barre syndrome among children and microcephaly in new borns has been reported coinciding with the time of the outbreak. Preliminary studies in Brazil suggest a correlation between the presence of virus in the blood and microcephaly in children. However, scientific studies are ongoing in Brazil to validate these claims.

Treatment:
There is no available drug or vaccine effective against Zika virus. Patients are generally advised to take rest and drink lot of water. Treatment, if required, is mainly supportive, including intravenous fluids and antipyretics. Anti-inflammatory drugs like Ibugesic need to be avoided till dengue positivity is completely ruled out.

vaccine effective against Zika virus
Laboratory Diagnosis: Zika virus can be tested in a BSL-2 facility. Testing for Zika virus should be done in pregnant women who have travelled to Zika endemic regions, or are symptomatic patients. Tests for dengue and chikungunya should also be done in view of similar symptoms.

  •  Acute phase (3 – 5 days): Detection of viral genome by RT-PCR in maternal serum and amniotic fluid.
  • Convalescent phase (≥5 days): Serology by testing IgM antibodies in blood. This is not the mainstay of diagnosis as cross reactivity with other flaviviruses is very high.

Plaque Reduction Neutralization Test (PRNT): This is a confirmatory diagnosis.
a. For testing acute phase samples:

  • National Institute of Virology, Pune has capacity to test the samples received during the acute phase of the disease by RT-PCR. The RT-PCR test available with NIV is standardized from published primers (Reference: Balm eta al: J Med Virol 2012; 84: 1501-5). These primers are developed from NS-5 non-structural gene of Zika virus, which is relatively conserved across the different strains.

b. For testing convalescent phase samples:

  • NIV & CDC are currently partnering on creating laboratory surveillance networks for enhancing diagnostic capabilities for high risk viral pathogens, under the Global Health Security (GHS) funds. As per this understanding, ICMR has requested CDC to provide Zika virus strains to NIV alongwith IgM ELISA diagnostic kits till in-house ELISA kits and PRNT tests are developed at NIV.

c. Others:

  • A training of DHR/ICMR VRDLs and concerned ICMR Institutes for Zika virus testing is shortly being organized by NIV, Pune. Tentative list of 20 labs have been identified for training.
  • ICMR has also issued an alert to several paediatricians under various networks of ICMR as well as the Viral Research & Diagnostic Laboratories to refer suspected samples for Zika testing at NIV, Pune. As of now, there are no diagnostic kits commercially available for testing of Zika virus in India.

Travel Advisory:
PAHO/WHO does not recommend any travel or international trade restrictions related to Zika virus outbreaks. Travelers are advised to take the suggested precautions to prevent mosquito bites. Pregnant women are being tracked in Brazil who is more likely to have babies with birth defects. CDC, Atlanta has released interim guidelines for physicians to advice pregnant women to avoid travel to ZIKA endemic regions region. Physicians are advised to enquire about recent travel in pregnant women.

Prevention and Control of Zika Virus disease:

  • Do
    Personal protective measures including use bed-net at home and hospital during Dengue/ Zika Virus fever to prevent mosquito bite so as to interrupt transmission. To avoid mosquito bite during day time wear full sleeved clothing and apply mosquito repellent. Eliminating household Aedes mosquito breeding sites. Integrated Vector Management (IVM) including chemical, biological and environmental vector control. Improving community awareness by effective IEC. For preventing/ controlling mosquito breeding. Cover all water tanks and containers with tight lids. Dispose & destroy all unused containers, junk materials, tyres, coconut shells etc. Empty, scrub & dry desert coolers every week before refilling. Use larvivorous fish in ornamental tanks at houses and hotels Observe weekly dry day- empty and scrub all containers holding water for a week like bird bath, flower vase, ant-trappers etc. and for managing fever use paracetamol for fever.
  • Don’ts
    Don’t allow water to stagnate in and around your house in coolers, buckets barrels, flower pots, bird baths, freeze trays, coconut shells etc. Don’t throw broken utensils, unused bottles, tins, old tyres and other junk materials here and there as Aedes mosquitoes breed in these objects during rainy season. Don’t use Aspirin to treat fever. There is no specific medicine for Zika Virus disease. Self-medication should be avoided and consult your doctor.

By:- Dr. Ashish Uniyal
Department of Agriculture.
Uttaranchal (P.G.) College Of Bio-Medical Sciences & Hospital